Grinding: An Insight into The New Era
نویسندگان
چکیده
As we enter a new millennium, society is faced with fresh challenges in every conceivable area. Through the speciality of forensic odontology, dentistry plays a small but significant role in identification of individuals. Cryogenic Grinding is the act of cooling or chilling a material and then reducing it to a small particle size. DNA is extracted from human teeth with the help of cryogenic grinding for PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) based forensic analysis. This technique provides with a high DNA yield of 30.9 micrograms DNA per tooth which is sufficient to provide the target DNA for more than 30,000 PCR from these samples which are too flexible or sensitive to be ground at ambient temperatures. This simple and rapid technique can be used to extract the DNA which is embedded in the hard tissues of the teeth. RNA can also be extracted by this method. This technique is advantageous over other techniques because it provides isolation of the samples in a closed system and in sterile conditions and also prevents cross contamination because the components of the apparatus can be easily cleaned and sterilized between samples. This versatile technique has evolved through many stages of using mortar and pestle to the recent homogenizer system and hence an important tool for further research in the field of forensic odontology.
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تاریخ انتشار 2015